马尾松毛虫安全区划分的动物多样性指数指标研究

通过比较广西宾阳县、宁明县、陆川县、临桂县、全州县马尾松毛虫安全区春季与夏季松林群落中的害虫种数、天敌种数、中性动物种数和益害比、动物多样性指数,认为划分马尾松毛虫安全区动物多样性指数的具体数值为≥2.300,据此进一步将安全区划分为基本稳定区、中等稳定区和高度稳定区.     

对朗乡林区土壤的评价和合理利用的建议

对朗乡林区所拥有的各类土壤进行评价并提出合理利用的建议。     

浙江省苗木花卉产业发展现状与对策

分析了浙江省花卉苗木产业、产值和规模居全国首位，区域分工日臻成熟，观赏苗木优势日趋稳固，科技支撑日益增强，产品结构调整加速等现状特点，指出了制约产业发展存在结构不合理，品种创新、保护力度不足、标准化程度低、信息服务体系不完善等问题，提出了控制适度规模、坚持科技创新、优化产品结构、坚持品牌创新、发展外向型产品和建立健全监管制度的对策和措施，推动浙江省花卉苗木由数量扩张型向质量效益型转变，朝理性化方向发展。     

温度、补充营养和体型大小对白蛾周氏啮小蜂成虫寿命的影响

寄生蜂成虫能否找到并寄生足够的寄主,是实现其最大生殖力的重要限制性因素之一[1],该因素与寿命直接相关,故寿命在进化中受到较大的自然选择压力,从而成为寄生蜂重要的适应性特征之一[2];在害虫生物防治实践中,寿命是决定寄生蜂控制寄主害虫能力的重要参数,是常用的评价天敌     

干旱地区枣粮间种“两大效益”的调查研究

通过对枣粮间作区和纯农作业区水土径流含沙量测定和收益情况调查,表明:枣粮间作区含沙量低于农作物区15.16%,土埂径冲刷损害轻微;枣粮间作区经济效益是纯农物区的4.7倍。     

月季快速繁殖技术研究

研究不同植物生长调节剂及质量浓度对带芽茎段分化、继代和生根的影响.结果表明:月季芽的分化培养基为M S+BA 1.0 m g.L-1+NAA 0.1 m g.L-1,不定芽的诱导率为80%;在M S+BA 1.0 m g.L-1培养基上继代增值的效果较好;NAA的生根效果优于IBA,以1/2M S+0.5 m g.L-1NAA最佳.     

森林防火3S平台的构建及其应用初探

介绍了3S系统的概念、特点及其在森林防火中的应用意义、国内外3S技术研究及应用进展;从实用性和可靠性、方便性和高效性、安全性和保密性、系统性和整体性、先进性和前瞻性等方面阐述了构建森林防火3S平台的原则;提出通过林火信息管理子系统、林火扑救指挥和实时监测子系统、林火预测预报子系统、林火设施的布局分析子系统、灾后评估及后处理子系统等的建立与应用,构建森林防火3S平台;并展望了3S技术在森林防火中的应用前景。     

石灰添加量对平菇生长发育的影响

以平菇‘新831’为试材,研究了不同石灰添加量对棉籽壳熟料栽培平菇的影响。结果表明:在棉籽壳中添加石灰能够提高培养料pH值、减低杂菌感染率、降低菌丝生长速度、减弱菌丝长势、延迟出菇时间,添加适量石灰可以提高平菇栽培生物学效率。石灰添加量为2%时,平菇栽培生物学效率最高。     

Hsa-miR-218 inhibits growth of cervical cancer HeLa cells by targeting to LASP1

AIM: To study the effect of hsa-miR-218 on cervical cancer HeLa cell growth and the underlying molecular mechanism.METHODS: The lentivirus expression vector pmiR-218 targeting to hsa-miR-218 was constructed. pmiR-218 was transfected into HeLa cells. The number of viable HeLa cells was counted by the method of Trypan blue exclusion. The inhibitory rate of cell activity was detected by WST-8 assay. The expression of LIM and SH3 protein 1(LASP1) at mRNA and protein levels was determined by real-time PCR and Western blot. The interaction between miR-218 and LASP1 was examined using a luciferase reporter assay.RESULTS: The lentivirus expression vector pmiR-218 targeting to hsa-miR-218 was constructed successfully and confirmed by DNA sequencing. Over-expression of miR-218 inhibited the activity of HeLa cells with the inhibitory rates of 15%, 26% and 65% at 24 h, 48 h and 72 h, respectively. The difference between transfection group and blank control/negative control group was statistically significant. The luciferase activity was reduced when co-transfection with miR-218 mimics and LASP1-3,UTR plasmid. The relative expression of miR-218 was increased after transfection with pmiR-218. Over-expression of miR-218 down-regulated the LASP1 expression at mRNA and protein levels by 25% and 75% respectively. Compared with blank control group and negative control group, the difference was statistically significant(P<0.05).CONCLUSION: pmiR-218 effectively inhibits the growth of HeLa cells in a time-dependent manner. miR-218 targets to the 3,UTR of LASP1, thus down-regulating the expression of LASP1 in HeLa cells.     

38株灵芝子实体抗肿瘤质量的生物评价

针对38株灵芝子实体样品,开展醇提物抑制He La、SK-OV-3肿瘤细胞增殖活性和三萜含量测定研究,对其抗肿瘤质量进行评价,筛选具有开发抗肿瘤产品潜力的优质菌株。实验结果表明,33号、34号2株灵芝对He La细胞和SK-OV-3细胞均具有较强的杀伤作用,对2株细胞增殖抑制IC50分别为16.55μg·m L-1、10.2μg·m L-1和31.9μg·m L-1、30.64μg·m L-1,可作为优质资源进行进一步开发。各菌株灵芝子实体的三萜含量在1.372 mg·g-1~6.184 mg·g-1之间,不同菌株子实体间三萜含量差异较大,细胞增殖抑制活性与三萜含量间不具有相关性,提示灵芝抗肿瘤原料质量评价宜考虑补充生物质量评价方法。